Human Growth hormone is a protein produced by the pituitary gland, a pea sized human gland situated beneath the brain. Human Growth hormone is among the hormones crucial for regular growth in children and teenagers, and it's also associated with managing metabolic process. In adults, human growth hormone deficit is very rare.
Human Growth Hormone production - Origin
Human growth hormone was extracted from human cadavers' brain until the early 1980's. The National Institute of Health created a programme in 1960 to obtain human growth hormone from the human cadavers. The entire process of getting growth hormone from the cadavers was costly and time consuming and in late 1970s it was discovered that a small risk of contracting Creutzfeldt-Jakob disease is associated with this process. As per the USA's National Endocrine and the Metabolic Diseases Information Services, in 1977, 28 instances of Creutzfeldt-Jakob illness were recorded amongst 7,700 individuals who were treated with human growth hormone. Development of the isolation and refinement technologies of HGH from cadavers did get rid of the danger of developing this Creutzfeldt-Jakob disorder.
Recombinant HGH Protein
Human Growth hormone is a protein made up of 191 amino acids. Each amino acid is encoded by a series of DNA. Recombinant DNA (rHGH) is created synthetically in the research laboratory. Sequences of DNA constructed till the whole protein is encoded. Subsequently, the recombinant DNA is injected into cells and that bulk develops the protein.
Various Human Growth Hormone productive Technologies
Inclusion Body Technology
The very first technique of producing rHGH was referred to as inclusion body technology. In this method E. coli bacteria mass produce rHGH, this builds up in tiny bodies within the bacteria. These small bodies are separated and the remarkably enriched rHGH is segregated and purified. The rHGH created by this technology varies by one additional amino acid in the end of the protein. Even though this rHGH was a marked improvement to cadaver extracted growth hormone, the additional amino acid triggered an allergic reaction in most individuals.
Protein Secretion Technology
In 2010, a more recent technology is employed to manufacture the majority of presently available rHGHs in the market and referred to as protein secretion technology, and its key advantage is the fact rHGH is released from E. coli and doesn't collect in inclusion bodies. This method enables less complicated purification of rHGH and the manufacture of rHGH which is just like human growth hormone.
Mouse Cell Technology
Producing rHGH applying mouse cell technology entails insertion of a DNA which encodes for human growth hormone in to the DNA in the mouse cells. The protein is developed in the mouse cells rather than E. coli bacteria in the research laboratory. Though more expensive at first, this technique creates rHGH that needs a lesser amount of treatment and purification. This technology also generates rHGH which is identical to HGH
Human growth hormone is vital for correct growth and development in children. Several children, even so, have issues that induce decreased HGH ranges. If these children go with no treatment method, they grow as abnormally shorter adults. This problem is dealt with by giving HGH, which nowadays is created by applying recombinant DNA (rDNA) technology.